亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>HUVECHUV-EC細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

HUV-EC細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

簡(jiǎn)要描述:HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):HUVEC
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:2065

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

ATCC® Number:CRL-1730™    Price:$280.00
DeBiosSesignations:HUV-EC-C

rum:afety Level:1

Shipped:frozen

Medium &See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:endothelial

Source:Organ: umbilical vein
Tissue: vascular endothelium
Disease: normal
Cell Type: endothelial


Cellular Products:factor VIII [23284]

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

Applications:transfection host (technology from amaxa)

Tumorigenic:No

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 9,11

D16S539: 11,12

D5S818: 11,12

D7S820: 8,12

THO1: 6,9.3

TPOX: 8,11

vWA: 16



Cytogenetic Analysis:This is a hypodiploid human cell line. The modal chromosome number was 45 occurring in 72% of cells counted. The rate of polyploid cells was 15.8%. All cells had monosomic N13 and the subclone with additional monosomic N15 predominates. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. Both X chromosomes appear normal.

Comments:Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. [23284]

The cells have a life expectancy of 50 to 60 population doublings.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Two to three times per week


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020



References:23284: Hoshi H, McKeehan WL. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. USA 81: 6413-6417, 1984. PubMed: 6333682
29192: Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915
33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed:8621443























產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美一区二区三区四区久久| 一区三区精品久久久精品| 中文字幕熟妇人妻av在线| 国产精品免费视频一区二区三区| 一区二区三区三级视频| 日韩无遮挡免费在线观看| 久久日韩一区二区三区| 欧美日韩综合精品无人区| 一起草视频在线观看视频| 中文字幕在线精品乱码| av 一区二区三区av| 海角社区av在线播放| 日韩欧美二区三区在线| 亚洲免费av一区二区| 日本熟女视频免费观看| 国产一级无码免费视频| 日韩一区二区三区91| 在线观看永久免费黄色| 国产精品一品二区三区在线观看| 日本束缚人妻一区二区三区| 亚洲最新精品一区二区| 日本写真视频一区二区三区| 日韩精品一区二区三区四区在线视频| 免费国产中文字幕黄网站| 蜜臀视频在线观看免费| 色婷婷狠狠久久综合中文一本| 日本精品在线一区二区| 青青久久精品国产亚洲av | 一区二区三区成人高清视频| 精品人妻少妇av一区二区| 麻豆精品国产免费av影片| av剧情在线观看免费| 亚洲av毛片在线免费播放| 日韩不卡免费一区二区三区视频| 黄色大全欧美在线观看| 亚洲综合久久五月天| 中文字幕人妻熟女在线| 久久国产成人精品免费看| 少妇性生活视频免费观看| 亚洲乱码一区二区免费版| 亚洲国产日朝欧美综合久久|