亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-2234SNU-449細(xì)胞, 人肝癌細(xì)胞

SNU-449細(xì)胞, 人肝癌細(xì)胞

簡(jiǎn)要描述:SNU-449細(xì)胞, 人肝癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫(kù)管理規(guī)范,提供 *培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-2234
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:1934

產(chǎn)品分類

Product Category

詳細(xì)介紹

SNU-449細(xì)胞, 人肝癌細(xì)胞(ATCC® CRL-2234)

Permits and Restrictions

View Restrictions

OrganismHomo sapiens, human
Tissue

liver

Product Formatfrozen
Morphologyepithelial; diffusely spreading cells
Culture Propertiesadherent
Biosafety Level2  [Cells contain Hepatitis B virus]
Diseasegrade II-III/IV,hepatocellular carcinoma
Age52 years
Gendermale
EthnicityAsian
Storage Conditionsliquid nitrogen vapor phase
Karyotypeaneuploid; modal number = 57
Derivation

SNU-449 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy.

Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum.
Clinical Data

52 years

Asian

male

SNU-449細(xì)胞, 人肝癌細(xì)胞

Comments

Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.

Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was predominantly compact and minor trabecular type.

The cultured cells contain a single or double nucleus.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
SubculturingProtocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days


Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
日韩一区中文字幕久久| av天堂在线观看网站| 国产精品久久久久久久久| 国产91精品系列在线观看| 久久日韩制服丝袜人妻| 亚洲欧美综合一区二区三区| 手机不卡在线观看av| 亚洲三级av在线播放| 美女呻吟被爽到高潮在线| 亚洲日本成人av在线观看| 老湿机午夜十分钟视频| 日韩一区二区三级电影| 色婷婷精品综合久久狠狠| 亚洲视频一区二区精品| 日韩爱视频一区二区| 日韩精品综合成人欧美| 日韩精品一区二区视频| 性感91美女白丝在线精品| 香蕉视频在线观看亚洲精品| 精品熟女少妇av免费观看| 日韩亚洲毛片全在线播放| 欧美成人日本在线播放| 91精彩啦在线看国产| 国产精品国产高清国产一区| 精品亚洲午夜久久久久| 欧美亚洲精品一区在线观看| 蜜桃av噜噜一区二区三| 国产传媒视频在线免费观看| 亚洲中文字幕婷婷在线| 国产又大又长又粗又硬又猛| 中文字幕一区精品日韩| 欧美人妻不卡一区二区久久| 日本免费一区二区三个| 国产三级黄色片免费看| 久久99久久精品视频国产| 国产精品一区在线免费看| 精品亚洲欧美日韩国产| 日韩精品国产自拍在线| 91中文在线视频播放| 国产精品超碰在线观看| 国产三级国产精品国产|