亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>人腫瘤細胞、癌細胞>CCL-220™COLO 320HSR細胞, 人結(jié)腸癌細胞

COLO 320HSR細胞, 人結(jié)腸癌細胞

簡要描述:COLO 320HSR細胞, 人結(jié)腸癌細胞
ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件

  • 產(chǎn)品型號:CCL-220™
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1638

產(chǎn)品分類

Product Category

詳細介紹

COLO 320HSR細胞, 人結(jié)腸癌細胞

COLO 320DM (ATCC® CCL-220™)

Organism  Homo sapiens, human

Tissue  colon

Product Format  frozen

Morphology  rounded and refractile

Culture Properties  mixed, adherent and suspension

Biosafety Level  1

Disease  Dukes' type C, colorectal adenocarcinoma

Age  55 years

Gender  female

Ethnicity  Caucasian

Storage Conditions

Karyotype  modal number = 24; range = 49 to 61.

The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.

Images

Clinical Data  55 years

Caucasian

female

Genes Expressed  serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone

Tumorigenic  Yes

Effects  Yes, in nude mice

Comments  The line is negative for CEA, CSAp and colon antigen 3.

The cells are weakly positive for keratins and vimentin

Complete Growth Medium  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


COLO 320HSR細胞, 人結(jié)腸癌細胞

Subculturing  Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.

1.Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.

2.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

3.Add appropriate aliquots of the cell suspension to new culture vessels.

4.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation  Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: Liquid nitrogen vapor phase

Culture Conditions  Temperature: 37°C

STR Profile  Amelogenin: X

CSF1PO: 11

D13S317: 11

D16S539: 11,12

D5S818: 12

D7S820: 9,12

THO1: 9

TPOX: 8,9

vWA: 15,18

Isoenzymes  ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Name of Depositor  GE Moore

References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美日韩成人精品一区二区| 亚洲精品午夜久久久av| 国产日韩欧美精品激情| 激情五月婷婷中文字幕| 国内揄拍国内精品少妇国| 欧美视频在线观看香蕉| 国产精品一级二区三区| 国产在线视频不卡一区| 在线天堂一区二区三区| 国产日韩手机在线不卡视频| 亚洲国产精品热久久网站| 亚洲一区二区精品999| 日本少妇人妻中文字幕| 国产av毛片一区二区| 伊人青草免费在线视频 | 亚洲一区二区视频在线播放| 国产剧情av色诱女教师| 日本人妻免费在线观看| 国产H精品在线观看| 欧美日韩亚洲1区2区| 亚洲男人天堂在线播放| 最新日本欧美一区二区| 人妻少妇被猛烈进入文字幕| 亚洲精品第一页中文字幕 | 青草草在线观看视频| 精品不卡一区二区三区| 国产av一区二区三区野战| 在线视频日韩欧美国产二区| 99久久免费看国产精品| 91成人国产综合久久精品| 美女丝袜诱惑国产91| 中文字幕日本精品人妻在线| 成人黄色动漫在线播放| 亚洲国产99在线精品一区| 日韩在线一区二区三区电影 | 国产女孩精品在线播放| 亚洲毛片高清一区二区三区| 成人黄片免费在线播放| 极品大胸美女被啪啪的高潮| 中文字幕亚洲精品熟女少妇| 日本熟女视频免费观看|