亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>人正常細胞>G HA-VSMC人血管平滑肌細胞

人血管平滑肌細胞

簡要描述:CRL-1999 T/G HA-VSMC 人血管平滑肌細胞,
原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,
提供的細胞株背景清楚,提供參考文獻和培養(yǎng)條件!

  • 產(chǎn)品型號:G HA-VSMC
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:2066

產(chǎn)品分類

Product Category

詳細介紹

CRL-1999 T/G HA-VSMC 人血管平滑肌細胞

ATCC® Number: CRL-1999™ Price: $264.00

Designations: T/G HA-VSMC

Depositors: RR Gifford

Bioped: frozen

Medium & Serum: See Propagation


Growth Propersafety Level: 1

Shipties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast


CRL-1999 T/G HA-VSMC 人血管平滑肌細胞

Source: Organ: aorta

Tissue: smooth muscle

Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applons: tranicatisfection host (technology from amaxa)

Reverse Transcript: N

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 12

D5S818: 12,13

D7S820: 9,10

THO1: 7,8

TPOX: 8

vWA: 16

Cytogenetic Analysis: This is a normal human cell line with a 46,XX karyotype. The modal chromosome number was 46, occurring in 90% of cells. The rate of polyploid cells was 0.8%. No structurally altered chromosomes were detected in 15 metaphases karyotyped. Both X chromosomes appeared normal.

Age: 11 months

Gender: female

Ethnicity: Caucasian

HeLa Markers: N

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, and incubate at 37C for 5 minutes. Add an equal volume of fresh medium, disperse the cells and centrifuge to pellet the cells. Resuspend the cells in fresh medium, aspirate and dispense into new flasks.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020



CRL-1999 T/G HA-VSMC





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
日本一区二区三区久久久| 性生活自制视频网站麻豆| 欧美福利免费在线视频| 91激情黑丝在线观看| 国产精品亚洲国产精品| 日本五十路亲子在线一区| 手机黄色av免费在线网址| 亚洲激情中文字幕av网| 国产午夜精品自拍视频| 久久人妻制服乱码中文字幕| 午夜视频在线观看麻豆| 欧美视频亚洲视频自拍视频| 久久精品国产av极品| 亚洲日日夜夜噜噜爽爽| 国产成人自拍视频网站| 久久精品色一情一乱一伦| 欧美激情日韩精品久久久| 熟妇人妻久久中文字幕麻豆网| 欧美日韩国产综合精品亚洲| 国产在线不卡中文字幕| 国产高清白丝免费在线观看| 欧美亚洲另类国产精品| 久久久久亚洲av成人| 欧美精品中出一区二区三区| 成人亚洲精品一区二区三区| 国产精品熟女亚洲av| 国产精致成人免费视频| 国产亚洲欧美精品久久久久| 国产亚洲精品一区在线| 亚洲各类熟女们中文字幕| 久久91亚洲精品久久91| 每日更新中文字幕粉嫩av| 传媒精品视频在线观看| 免费视频观看在线一区二区三区| 年轻的母亲韩国三级| 人妻av一区二区三区| 国产三级精品三级精品在一区| 熟女精品国产一区二区三区 | 精品裸足人妻少妇二区三区 | 公交车上没穿内裤被插高潮不断| 欧美日韩一区二区综合在线视频|