亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CCL-9.1NCTC 1469 小鼠正常肝細(xì)胞

NCTC 1469 小鼠正常肝細(xì)胞

簡(jiǎn)要描述:CCL-9.1 NCTC 1469 小鼠正常肝細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CCL-9.1
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:3750

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-9.1 NCTC 1469 小鼠正常肝細(xì)胞 的詳細(xì)介紹

CCL-9.1 NCTC 1469 小鼠正常肝細(xì)胞

ATCC® Number:CCL-9.1™  Price:$329.00
Designations:NCTC clone 1469 [derivative of NCTC 721]

Depositors:VJ Evans

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:epithelial


Source:Organ: liver
Strain: C3H/An
Disease: normal


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CCL-9.1

Isolation:Isolation date: April, 1952

Virus Susceptibility:Vesicular stomatitis virus

Human poliovirus 1



Antigen Expression:H-2k

Cytogenetic Analysis:modal number = 41; range = 38 to 86.

Two marker chromosomes present: One metacentric with one pair of arms having achromatic gaps near centromere; and an acrocentric with salites.



Age:newborn

Gender:male

Comments:Tested and found negative for ectromelia virus (mousepox).

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh medium and dislodge the cells from the floor of the flask. Aspirate cells and dispense the suspension into new flasks.

A standard trypsinizaton may be used if desired.

The culture medium in heavy cultures may be turbid in appearance and and give the gross impression of bacterial contamination.

It is characteristic of this line to shed viable cells into the medium, thus rendering the medium turbid. The shed cells are viable and may be used to initiate new cultures.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: 3 times per week


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2040



References:22167: Hobbs GL, et al. Establishment of a clone of mouse liver cells from a single isolated cell. J. Natl. Cancer Inst. 18: 701-707, 1957. PubMed: 13502690

26070: Evans VJ, et al. The growth in vitro of massive cultures of liver cells. J. Natl. Cancer Inst. 12: 1245-1265, 1952. PubMed: 14939026

26074: . . J. Natl. Cancer Inst. 23: 823, 1959.

26076: . . J. Natl. Cancer Inst. 27: 29, 1961.

26078: Evans VJ, et al. Studies on culture lines derived from mouse liver parenchymatous cells grown in long-term tissue culture. Cancer Res. 12: 261-266, 1958. PubMed: 13523589

26079: . . Fed. Proc. 17: 967, 1958.

26081: Westfall BB. Characterization of cells in tissue culture. Natl. Cancer Inst. Monogr. 7: 147-158, 1962. PubMed: 14006338



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
91福利免费在线看| 午夜体内射精免费视频| 18禁污污网站国产| 国产爆操美女在线观看| 2021亚洲精品午夜精品国产| 久久精品国产成人综合| 一区二区三区国产精品乱码| 熟女俱乐部五十路六十路| 日本毛茸茸的丰满熟妇| 欧美 日本国产一区| 欧美+亚洲+精品+三区| 一级亚洲国产日韩欧美| 日韩人妻av免费电影| 久久 久久国内精品亚洲| 久久精品国产亚洲av麻豆她| 在线观看国产自拍精品| 97人妻精品一区二区三区六| 国产日产精品一区二区三区四区| 性生活真实视频免费看| 婷婷五激情五月激情片| 亚洲婷婷综合久久一区二区| 欧美丰满熟妇视频在线| 国产精品日韩欧美亚洲| 欧美日本国产高清不卡| 天堂社区人妻在线亚洲| 中文字幕一区二区久久综合| 日本中文字幕激情在线| 91国内精品手机在线高清| 国产午夜激情在线播放| 成年人的黄色大片网站| 亚洲综合久久五月天| av中文字幕啊嗯不要| 久久亚洲av麻衣北条麻妃 | 亚洲一区二区三区日本久久| 国产久精品热看久品热久热| 国产伊人久久综合网| 区二区三区毛片乱码免费| 美女午夜福利一区二区| 久久久久国产综合精品| 国产精品一级片一区二区| 国产精品自拍激情在线观看|