亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-1503STO 小鼠胚成纖維細(xì)胞系

STO 小鼠胚成纖維細(xì)胞系

簡要描述:CRL-1503 STO 小鼠胚成纖維細(xì)胞系
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1503
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2096

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1503 STO 小鼠胚成纖維細(xì)胞系 的詳細(xì)介紹

CRL-1503 STO 小鼠胚成纖維細(xì)胞系

ATCC® Number:CRL-1503™  Price:$289.00
Designations:STO

Depositors:G Martin

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:fibroblast


Source:Organ: embryo
Strain: SIM
Cell Type: fibroblast


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1503

Isolation:Toronto Ontario, Canada

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Age:embryo

Comments:The STO cell line was derived by A. Bernstein, Ontario Cancer Institute, Toronto, Canada from a continuous line of SIM mouse embryonic fibroblasts. The population which was selected for 6-thioguanine and ouabain resistance is sensitive to HAT medium and is HPRT negative. The cell line is used routinely to prepare feeder layers by irradiation or mitomycin C treatment. Such populations are then employed for maintenance of stock teratocarcinoma stem cells (see ATCC CRL-1535 and CRL-1566) in the undifferentiated state.The cells have been tested and found negative for ectromelia virus (mousepox).

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:10 is recommended
      Medium Renewal: 2 to 3 times per week



Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

irradiated to be used as feeder cells:ATCC 56-X



References:1070: Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416

21874: . Teratomas and differentiation. New York: Academic Press; 1975.

22387: . . Cell 6: 467-474, 1975.

22701: Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲精品一品区二品区三区| 色婷婷一区二区三区四| 亚洲国产精品一区二区电影| 激情五月开心五月麻豆| 国产剧情av色诱女教师| 国产av一区最新精品麻豆| 黄色片在线观看中文字幕| 日本一区二区不卡视频在线播放| 亚洲男女尻逼片视频网站| 国产精品一区二在线观看| 久久这里只有精品热免费| 日本欧美国产一区二区| 国产av剧情在线免费观看| 午夜福利日本一区二区| 亚洲综合中文字幕久久网址| 欧美性大片一区二区三区| 特黄一级黄色大片免费看| 亚洲国产精品欧美激情| 久久夜色精品国产高清不卡| 传媒视频在线免费观看| 黄色片一区二区三区四区| 风韵丰满熟妇啪啪老熟女| 亚洲一区二区三区精品福利| 女子张开腿让男人捅爽| 尤物视频官网在线观看| 国产精品一级自拍视频| 中文字幕乱码亚州精品一区| 一区二区三区都市激情| 日本一区二区中文字幕视频| 青青草视频在线好好热| 国产精致成人免费视频| 亚洲综合av婷婷激情| 一本色桃子精品久久中文字幕| 亚洲欧美国产成人在线| 日本特黄高清免费大片| 日韩人妻中文字幕在线视频| 四虎成人免费永久视频| 女性裸体无遮挡啪啪网站| av黄色成人在线观看| 日本2区3区视频在线观看| 濑亚美莉在线观看一区二区三区|