亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞

CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞

簡(jiǎn)要描述:CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|。細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:2160

產(chǎn)品分類

Product Category

詳細(xì)介紹


CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞


ATCC® Number:CRL-2268™Price:$329.00
Designations:BE(2)-C

Depositors:JL Biedler

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:neuroblast
CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞


Source:Organ: brain

Disease: neuroblastoma

Derived from metastatic site: bone marrow



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Restrictions:BE(2)-C was deposited at the ATCC by June L. Biedler, Memorial Sloan-Kettering Cancer Center. BE(2)C is distributed for academic research purposes only. Memorial Sloan-Kettering releases the line subject to the following: 1.)BE(2)-C or its products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of BE(2)-C including any use by a for-profit entity must first be negotiated with Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Isolation:Isolation date: November, 1972

DNA Profile (STR):Amelogenin: X

CSF1PO: 10

D13S317: 11

D16S539: 9,11

D5S818: 12

D7S820: 9,10

THO1: 6

TPOX: 11

vWA: 18



Age:2 years

Gender:male

Comments:BE(2)-C is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.

BE(2)-C cells have a reported saturation density of greater than 5 X 10 exp5 cells/sq cm.

The cells grow as clusters of flattened neuroblastic cells with occasional fine cell processes (neurites).

Unlike the parent line, they generally do not detach and float.



Propagation:ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C



Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase



Doubling Time:18 hrs

Related Products:recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-2271























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美日韩国产看片一区二区| 人人看男人的天堂东京| 亚洲国产高清国产拍精品| 一区二区亚洲免费的视频| 福利午夜福利在线观看| 亚洲综合激情另类专区| 亚洲日本香蕉视频观看视频| 免费观看亚洲视频一区二区三区 | 婷婷五激情五月激情片| 色六月婷婷六月久久六月| 尤物视频最新在线观看| 国产女片xb内射在线| 久热99在线视频免费观看| 日韩国产在线一区二区| 人妻少妇被粗大爽av| 亚洲综合国产一区二区| 亚洲精品一区二区三区三州| 欧美日韩专区一区二区三区| 国产91在线拍揄自揄| 中文在线在线天堂中文| 亚洲欧美日韩精品麻豆| 日本在线观看免费高清| 国产中文字二暮区2021综合| 最新亚洲国产高清激情| 久久夜色噜噜噜av一区| 亚洲av日韩高清在线观看| 日韩精品少妇一区二区| 伊人久久亚洲精品综合| 韩国福利短片在线观看| av欧美激情在线观看| 国产大学生吞精在线视频| 婷婷五激情五月激情片| 日本高清不卡在线一区二区| 国产三级黄在线观看| 日本午夜精品在线观看| 国产在线精品成人欧美| 四虎在线观看最新免费| 日韩少妇人妻一区二区| 成年黄网站免费视频大全| 日本熟人妻中文字幕在线| 一本在线不卡中文字幕|